Effect of Anabolic Androgenic Steroid on Body Weight and Its Protection by Punica Granatum in Mice Model

Humaira  Ali; Uzma Tipu Awan; Muhammad Sarwar Zia; Khadija  Qamar

doi:10.29054/apmc/2017.218

Humaira Ali Assistant Professor, Anatomy HITEC-IMS, Taxila Cantt, Taxila
Uzma Tipu Awan Principal Medical Officer, Head of Radiology Department, Margla Hospital, Taxila
Muhammad Sarwar Zia Assistant Professor, Anatomy KMSMC, Sialkot
Khadija Qamar Professor & Head of Anatomy Army Medical College, Rawalpindi

DOI: https://doi.org/10.29054/apmc/2017.218

Keywords: Punica granatum, body weight, Nandrolone Decanoate

Abstract

Objectives: To determine the effect of anabolic androgenic steroid (AAS) on body weight and observe the protective role of two forms of Punica granatum (Pomegranate). Place and duration of study: Department of Anatomy, Army Medical College (AMC), Rawalpindi in collaboration with National Institute of Health (NIH), Islamabad from May 1^st to June 30^th, 2015. Methodology: In this study, 40 healthy mice (BALB/c-strain) having average weight of 25-30 gms were divided into four groups, each group having five males and five females. Ten animals were kept in one cage. Mice were fed with NIH standard laboratory diet pellets for eight weeks with water ad libitum. Group A which served as a control group received only lab diet. Groups B, C and D served as experimental groups. These three experimental groups were injected ND (Nandrolone decanoate) (1 mg/100 gm of body weight), intramuscularly (I/M), in the back limb (once weekly) for 8 weeks. Experimental group C also received pomegranate juice (PJ) (at the dose of 3ml/kg of body weight) via gastric tube daily for 8 weeks and experimental group D received pomegranate peel extract (PPE) (at the dose of 200mg/kg of body weight) through gastric tube daily for 8 weeks. Net body weight gain of animals was recorded by weighing the animals at the start and end of the study. The results of all the four groups were compiled and compared with each other. Results: The weights of animals were increased considerably in experimental groups B, C and D. No significant improvement was seen in pomegranate administered experimental groups C and D. Conclusion: Punica granatum has no protective role on steroid’s induced total body weight gain.