Preserving Hepatic Glycogen Stores: Investigating the Therapeutic Influence of Vitamin E in Alcoholic Liver Injury-Induced Intracellular Carbohydrate Depletion

Therapeutic Influence of Vitamin E on Alcohol-Induced Hepatic Glycogen Depletion

Abstract

Objective: The study aimed to explore and evaluate the effectiveness of Vitamin E in preventing or minimizing glycogen depletion in hepatocytes associated with alcoholic liver damage. Study Design: Experimental study. Settings: The study was conducted in the department of anatomy and animal facility of Peshawar Medical College, Peshawar Pakistan. Duration: February 2022 to April 2023. Methods: The study included eighteen male domestic rabbits (Oryctolagus cuniculus), classified according to the duration of the experiment. Rabbits in "Category E8" were exposed to an 8-week time frame, while those in "Category E4" experienced a 4-week experimental duration. Each classification was further segmented into three sections: "Control Group A" which was administered normal saline as a placebo; "Experimental Group B" received a 30% ethanol solution in distal water (30ml per kg/day) through a nasogastric tube; and "Experimental Group C" treated with a 30% ethanol solution in distal water (30ml per kg/day) administered along with "Vitamin E" (50mg dissolved in 2ml distal water per kg/day). Liver tissue specimens from all subjects underwent staining with PAS stain to quantify glycogen (carbohydrates) in hepatocytes. Results: A statistically significant variance in glycogen depletion within hepatocytes was noted between animals treated with vitamin E and those not treated with vitamin E in both E4 and E8 categories. Nonetheless, vitamin E treated rabbits exhibited higher glycogen levels compared to their non-vitamin E treated counterparts. Conclusion: In the realm of alcohol-induced liver damage, vitamin E provides the expected protective benefits by safeguarding glycogen (carbohydrate) stores within hepatocytes.