Effect of Plateletpheresis on Platelet Count and Mean Platelet Volume in Healthy Donors

Abstract

Background: Plateletpheresis is a process by which platelets are extracted from the donor by a device which returns other portions of the blood to the donor. This process yields 8 times more platelets than platelet derived from whole blood. Currently, there are discrepancies in the reports on the extent of the reduction in platelet count after platelet donation by apheresis, and its impact on mean platelet volume (MPV). Objective: To Assess the effect of plateletpheresis on platelet count and mean platelet volume after platelet donation. Study Design: Cross-sectional study. Settings: Department of Hematology University of Children Health, Lahore Pakistan. Duration: Six months from January 2022 to June 2022. Methods: The procedure of plateletpheresis was done on 30 donors and effect of plateletpheresis procedure on post procedure Platelet count and MPV was observed. Results: In this study, 65 donors were included; all were healthy male donors, with mean weight 73.27 ± 11.38 Kg. Among 65 male donors, pre donation platelet levels showed that 12.3% donors had platelet level <200, 58.5% had platelet levels of 200-350 and remaining 29.2% had platelets level >350. Regarding pre donation mean platelet volume levels, 46.1% had mean platelet volume levels in the range of 5-8, 30.8% donors had mean volume of 8.1-12 and 23.1% had 12.1-15 mean platelet levels. After 1 hour of donation post platelet levels and mean platelet volume of donors were measured. Changes in platelet count were observed as 18.5% donors had <200, 49.2% had platelet levels of 200-350 and 32.3% donors had platelet levels of >350. Mean platelet volume after donation of 36.9% had 5-8, 27.7% donors had mean volume of 8.1-12 and 35.4% had 12.1-15 mean platelet volume levels. Mean pre and post donation platelet levels of donors was 285.7±54.41 and 180.47±45.17 respectively. Mean pre and post donation platelet volume was 8.67±1.43 and 13.60±2.90 respectively. The difference was statistically significant for platelet levels as well as mean platelet volume (p-value<0.001). Conclusion: This study concluded that platelet donation by apheresis although reduces both platelet count and MPV in donors, which is detrimental to the purposes of the procedure but decrease in platelet count after plateletpheresis procedure was more significant and there was minor change in MPV which is not significant.