Pattern of Hemoglobinopathies And Thalassemia in Children by Using HPLC At LUMHS Jamshoro Hyderabad Sindh

  • Sadia Abbasi Consultant Haemotologist, Pathology Department Liaquat University of Medical and Health Sciences, Jamshoro Pakistan
  • Fahmeena Qadri Consultant Haemotologist, pathology department Liaquat University of Medical and Health Sciences, Jamshoro Pakistan
  • Maria Jawed Assistant Professor Bhitai Dental Medical College MirpurKhas Pakistan
  • Ikram Din Ujjan Professor of Pathology Liaquat University of Medical and Health Sciences, Jamshoro Pakistan
  • Saad Abbasi MS (General Surgery) Liaquat University of Medical and Health Sciences/Jamshoro Pakistan
  • Kulsoom Jawed Assistant Professor Community Medicine Dow international Medical College, DUHS, Karachi Pakistan
Keywords: Hemoglobinopathies, HPLC technique, Children


Objective: To determine the frequency of Hemoglobinopathies in children by using HPLC technique in Hyderabad Sindh. Study Design: Descriptive study. Duration: During 2015 and 2017. Methodology: All children aged below 12 were selected to diagnose thalassemia and haemoglobinothies. EDTA anticoagulant was used to collect a sample of 5 ml blood (intravenous). Red cell indices were evaluated on an autonomous sysmex XN1000 i. hematology analyzer. The HPLC technique was applied to evaluate the hemoglobin in various types such as A2, A, D, S, F and further types. Results: Patients’ mean age was 8.11+03.36 years. Thalassemia major and minor were highly common as; 36.5% (n=301) and 47.5% (n=301) respectively, after that Sickle cell disorder and  trait, Hb-D, Hb-C,  Hb-D disease and В-thalassemia with compound heterozygous, with percentages of 1.3%, 7.6%, 0.7%, 1.7%, 0.7% and 3.3% respectively, whereas Hb-E disorder was also seen in 2 subjects. Conclusion: We concluded that thalassemia minor, thalassemia major, Sickle cell trait, HbD & HbC trait, and HB-D were most common haemoglobinopatheis in children. HPLC is an excellent diagnostic tool to directly identify the haemoglobin variants in quantification of abnormal and normal haemoglobin fractions with a high precision.