Bacterial Isolates of Infected Wounds & Their Sensitivity to Antibiotics

Abstract

Background: Infection of the wounds has got definite role in delaying wound healing and development of chronicity. Various studies have been carried to identify & study the pattern of pathogens present in infected wounds and efficacy of antibiotics against these organisms. Objectives: To identify the most common pathogens involved in infections of the wounds after surgical procedures, patterns of their sensitivity and resistance to the different antibiotics. Setting & Duration: The study was carried out in Surgical Unit (3) of Lahore General Hospital, Postgraduate Medical Institute (PGMI) Lahore for the period of Six months from October, 2016 to March, 2017. Methodology: Wound swabs were collected using sterile cotton swabs after obtaining consent from patients. The isolated samples were identified by using standard parameters. Kirby baur disk diffusion method used to determine sensitivity. Tube dilution method was used for minimum inhibitory and bactericidal concentration. Results: Total 150 bacterial isolates were collected from infected wounds whereas 145(96.67%) were culture positive & 5(3.33%) were bacteriologically sterile. The most common bacteria were Staphylococcus aureus 47(32.4%) followed by Escherichia coli 29(20%), Proteus species 23(16%), Coagulase Negative Staphylococci 21(14.5%), Klebsiella pneumoniae 14(10%) and Pseudomonas aeruginosa 11(8%). All isolates showed high frequency of resistance to Ampicillin, penicillin, cephalothin and tetracycline. Conclusions: Staphylococcus Aureus has been found as the most common pathogen involved in wound infection. The antibiotics belonging to the group of Cephalothin, Ampicillin, Penicillin and Tetracycline are the least effective against pathogens isolated from infected wounds whereas Piperacillin alongwith Tazobactum; Amikacin & Norfloxacin are the most effective antibiotics against those pathogens.